Standard Caco-2 cell culture medium: EMEM (EBSS) + 2mM Glutamine + 1% Non-Essential Amino Acids (NEAA) + 10% to 20% Foetal Bovine Serum FBS / FCS.
HBSS (10X) buffer: Adjust to pH ~ 7.4, autoclave and store at 4°C until use.
CaCl2 2H2O (100 mM), autoclave and store at 4°C until use.
MgCl2 2H2O (50 mM), autoclave and store at 4°C until use.
Ethanol (70%).
Lucifer Yellow Stock 1mg/mL (SIGMA #L0259/#62642).